Rheumatoid factor‐like reactants in sera proven to transmit non‐A, non‐B hepatitis: A potential source of false‐positive reactions in non‐A, non‐B assays

Hiroyuki Shiraishi, Harvey J. Alter, Stephen M. Feinstone, Robert H. Purcell – 1 March 1985 – Convalescent phase non‐A, non‐B (NANB) human and chimpanzee sera were utilized in a solid‐phase radioimmunoassay (RIA) in an attempt to identify a specific NANB antigen in human plasma, plasma‐derived pellets and NP‐40 disrupted pellets proven to transmit NANB infection to chimpanzees. RIA reactivity was noted in 5 of 8 pedigreed NANB infectious plasmas, but did not appear to be virus‐specific.

Epidemiology and clinical impact of hepatitis D virus (delta) infection

Ira M. Jacobson, Jules L. Dienstag, Barbara G. Werner, Doreen B. Brettler, Peter H. Levine, Isa K. Mushahwar – 1 March 1985 – We tested sera collected between 1976 and 1984 from 362 persons in a variety of epidemiologic categories with acute and chronic hepatitis B and from 76 hemophiliacs and drug addicts with hepatitis B antibodies for hepatitis D markers.

T lymphocyte sensitization to hbcag and T cell‐mediated unresponsiveness to HBsAg in hepatitis B virus‐related chronic liver disease

Sandro Vento, John E. Hegarty, Alfredo Alberti, Charles J. O'brien, Graeme J. M. Alexander, Adrian L. W. F. Eddleston, Roger Williams – 1 March 1985 – Using a newly developed indirect T lymphocyte migration inhibition test, cell‐mediated immunity to HBsAg and HBcAg was directly and simultaneously examined in a total of 21 patients with HBsAg‐positive chronic liver disease (CLD), and in seven subjects whose sera contained anti‐HBs (2 previous acute hepatitis B; 4 hepatitis B vaccine recipients and 1 chronic active hepatitis).

Acetaminophen‐Induced hepatotoxic congestion in mice

Robin M. Walker, William J. Racz, T. Francis McElligott – 1 March 1985 – Acetaminophen‐induced (750 mg per kg p.o.) hepatotoxicity in mice is characterized by hepatomegaly and massive centrilobular congestion which precede the appearance of necrosis. The vascular changes are correlated with the morphologic features using liver hemoglobin content to quantitate erythrocyte sequestration, and hematocrit measurements and 125I‐albumin injections to determine plasma and blood volume.

Experimental laser phototherapy of the morris 7777 hepatoma in the rat

Stephen Holt, John Tulip, Douglas Hamilton, Judith Cummins, Anthony Fields, Catherine Dick – 1 March 1985 – The tumoricidal effect of the activation of hematoporphyrin derivative (HpD), by an argon‐ion‐dye‐laser (wavelength 630 nm), was investigated in the Buffalo rat bearing subcutaneous implants of the Morris 7777 hepatoma. Tumor growth was monitored by measuring the tumor volume with constant force callipers. In control animals and those that were pretreated with HpD alone (10 or 20 mg per kg by i.p.

Therapeutic efficacy of glucan in a murine model of hepatic metastatic disease

David L. Williams, Edward R. Sherwood, Rose B. McNamee, Ernest L. Jones, Nicholas R. Di Luzio – 1 March 1985 – Glucan, a particulate β‐1,3‐polyglucose immunomodulator, was evaluated for its ability to modify hepatic metastases and survival in mice with reticulum cell sarcoma. Sarcoma M5076 cells were injected subcutaneously (1 × 105 cells) into syngeneic C57BL/6J male mice. On Day 20, histopathological studies indicated the presence of hepatic micrometastases. At this time, glucan (0.45 mg per mouse) or dextrose was administered intravenously.

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