David E. Moody, Lorna A. Taylor, Edward A. Smuckler – 1 May 1985 – We have used an immunohistochemical approach to study the lobular distribution of constitutive liver microsomal cytochrome P‐450. Cytochrome P‐450 isolated (10.3 nmoles per mg protein) from hepatic microsomes from untreated, mature male Sprague‐Dawley rats was used to produce antisera in rabbits.
Marie‐Laurence Roquet, Elie‐Serge Zafrani, Jean‐Pierre Farcet, Félix Reyes, Yvon Pinaudeau – 1 May 1985 – Pathological lesions of the liver were studied in 14 cases of hairy cell leukemia, a rare hematologic neoplasm involving numerous organs. Tumor infiltration of hepatic parenchyma was observed in all cases. Tumor cells were easily recognized by their cytological features, namely their “halo” appearance consisting of a clear rim of abundant cytoplasm surrounding uniform round or slightly indented nuclei.
Rafael Esteban, Maria Buti, Rosendo Jardi, Jamie Guardia, Ding‐Shinn Chen, Ming‐Yang Lai, Juei‐Low Sung, George Papevangelou, Nicolaos Tassopoulos, Anastasia Roumeliotou‐Karayannis, Clive Richardson – 1 May 1985
Daniel Shouval, Dan Eilat, Rolf I. Carlson, Ruth Adler, Nelly Livni, Jack R. Wands – 1 May 1985 – A library of murine monoclonal antibodies reactive with human hepatoma cells was generated following immunization of Balb/c mice with an intact cloned human hepatoma cell line, designated PLC/PRF/5‐NR. We report the characterization of one such IgG2a antibody, designated anti‐PLC1.
Thomas B. Leonard, D. Gwyn Morgan, John G. Dent – 1 May 1985 – Cimetidine has been shown to protect against acetaminophen‐mediated hepatotoxicity in both rats and mice. In contrast to cimetidine, ranitidine recently has been determined to potentiate the hepatotoxic action of acetaminophen in Fischer 344 rats. The present studies were designed to characterize this ranitidine‐acetaminophen interaction. Acetaminophen administration (750 mg per kg, p.o.) to F344 rats produced maximal hepatic necrosis, 24 hr after treatment, as assessed by SGPT activity and histopathology.
Hyam L. Leffert, Dale B. Schenk, Jeffrey J. Hubert, Harold Skelly, Mark Schumacher, Reginald Ariyasu, Mark Ellisman, Katherine S. Koch, Gilbert A. Keller – 1 May 1985
Christine E. Bear, Connie N. Petrunka, Steven M. Strasberg – 1 May 1985 – Chloride is the major inorganic anion in bile but its mechanism of passage from blood to bile is uncertain. Specific membrane channels account for most net inorganic anion flux in other cell types such as the proximal tubular cell and red blood cell; disulfonic stilbenes inhibit anion movement through these channels. Therefore, we have sought the presence of similar channels in the hepatocyte.
Gary E. Sanders, Robert P. Perrillo – 1 May 1985 – The potential immunoregulatory effects of rosette inhibitory factor (RIF) were evaluated using lymphocyte subpopulations defined by monoclonal antibodies (OKT4, OKT8). Initial experiments indicated that RIF inhibits E‐rosette formation by T cells that provide helper/inducer function (OKT4+). Subsequently, experiments were done to assess if RIF had an inhibitory effect on in vitro immunoglobulin and anti‐HBs production. These studies used peripheral blood mononuclear cells from convalescent and hepatitis B‐vaccinated donors.
Richard J. Kroeger, Roberto J. Groszmann – 1 May 1985 – Present investigations support major contributions from increases in both portal blood flow and portal vascular resistance in the mechanism that maintains portal hypertension. β‐Adrenergic blockers have been shown to reduce the elevated portal blood flow component. The possibility that nitroglycerin administration could reduce the elevated portal vascular resistance component is investigated here. Portal hypertension was induced in rats by a calibrated constriction of the portal vein.