Effects of dexamethasone on albumin and collagen gene expression in primary cultures of adult rat hepatocytes

Douglas M. Jefferson, Lola M. Reid, Marie‐Adele Giambrone, David A. Shafritz, Mark A. Zern – 1 January 1985 – To further our studies on collagen gene expression, we have evaluated the molecular basis for the finding that steroids decrease collagen synthesis in cultured hepatocytes. We studied the effects of dexamethasone on primary cultures of adult rat hepatocytes grown on tissue culture plastic in either serum‐supplemented medium or a serum‐free hormonally defined medium.

Insulin action in cirrhosis

R. Taylor, R. J. Heine, J. Collins, O. F. W. James, K. G. M. M. Alberti – 1 January 1985 – In vivo insulin sensitivity and adipocyte insulin binding and action were assessed in 16 patients with histologically proven hepatic cirrhosis and 11 age‐, weight‐ and sex‐matched normal control subjects. The cirrhotic group displayed impaired oral glucose tolerance, despite an exaggerated serum immunoreactive insulin response, and in vivo insulin resistance as assessed both by the euglycemic hyperinsulinemic clamp and the glucose‐insulin infusion techniques.

The merck index. An encyclopedia of chemicals, drugs, and biologicals. Tenth Edition. Martha Windholz, Editor; Susan Budavari, Co‐Editor; Rosemary F. Blumetti, Associate Editor, Elizabeth S. Otterbein, Assistant Editor. 2, 067 pp. (including tables and in

Effect of chronic ethanol ingestion on hepatic and intestinal acyl coenzyme A:Cholesterol acyltransferase and 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase in the rat

F. Jeffrey Field, John S. Boydstun, Douglas R. Labrecque – 1 January 1985 – Two groups of rats were pair‐fed diets in which 36% of the calories were provided by either ethanol or dextrimaltose. After 60 days on these liquid diets, rats fed ethanol were significantly smaller than control rats fed dextrimaltose. Serum cholesterol levels in ethanol‐fed animals were 20% higher than control rats. Cholestasis was not observed histologically, and serum alkaline phosphatase and bilirubin levels were the same in both groups.

Hepatitis B Markers in United States Drug Addicts with Special Emphasis on the Delta Hepatitis Virus

Antonio Ponzetto, Leonard B. Seeff, Zelma Buskell‐Bales, Kamal G. Ishak, Jay H. Hoofnagle, Hyman J. Zimmerman, Robert H. Purcell, John L. Gerin – 1 November 1984 – Hepatitis B virus and hepatitis delta virus co‐infection in drug addicts has been well described in Europe, the latter agent appearing to have been introduced there in the mid‐1970's. Currently, similar data are scanty among United States addicts.

Regulation of Low Density Lipoprotein Receptor Function in a Human Hepatoma Cell Line

Alan M. Leichtner, Monty Krieger, Alan L. Schwartz – 1 November 1984 – Low density lipoprotein (LDL) processing was investigated in a human hepatoma‐derived cell line, Hep G2. Hep G2 cells bound, internalized and degraded LDL via a saturable, high affinity (Kd ∼ 2 x 10−8M) pathway similar to that present in other mammalian cells. Although 80% of the uptake and degradation of 125I‐LDL was inhibited by 40‐fold excess native LDL, the same concentration of methylated LDL, which cannot bind to LDL receptors, had virtually no effect on processing.

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