Spironolactone‐ and Canrenone‐Induced Changes in Hepatic (Na+, K+)ATPase Activity, Surface Membrane Cholesterol and Phospholipid, and Fluorescence Polarization in the Rat

Philip B. Miner, Michael Sneller, Synda S. Crawford – 1 January 1983 – We studied changes in hepatic membrane (Na+,K+)ATPase activity and membrane lipids induced by canrenoate, the water‐soluble congener of canrenone, the active metabolite of spironolactone. (Na+,K+)ATPase activity was decreased after canrenoate in a dose‐ and time‐dependent manner.

Detection of Regan Variant Type of Alkaline Phosphatase Isoenzyme in Liver Tissue of Indian Childhood Cirrhosis

S. R. Parekh, B. D. Patel, S. R. Damle, G. M. Thanki, D. Khutti – 1 January 1983 – Alkaline phosphatase (ALP) isoenzyme composition was studied in sera and liver from patients with Indian childhood cirrhosis (ICC). A typical pattern consisting of a fast‐moving anodal preliver band with slower moving diffuse liver and placental bands followed by an intestinal band was consistently observed in sera of patients in all stages of ICC and in pregnant mothers of index ICC patients. ICC liver ALP was relatively heat‐stable and inhibited by L‐phenylalanine and L‐leucine.

Vesicular Transport of Horseradish Peroxidase during Chronic Bile Duct Obstruction in the Rat

Richard H. Renston, Gyula Zsigmond, Robin A. Bernhoft, Susan J. Burwen, Albert L. Jones – 1 January 1983 – The vesicular transport system for biliary secretion of plasma‐derived proteins was investigated in rats with chronic bile duct obstruction. Horseradish peroxidase, previously demonstrated to be a suitable tracer for vesicular transport, was employed in these studies.

Hepatocyte Membrane‐Bound IgG and Circulating Liver‐Specific Autoantibodies in Chronic Liver Disease: Relation to Hepatitis B Virus Serum Markers and Liver Histology

Riccardo Meliconi, Federico Miglio, M. Valeria Stancari, Mario Baraldini, G. Francesco Stefanini, Giovanni Gasbarrini – 1 January 1983 – Hepatocytes isolated from 101 biopsies were examined for membrane‐bound IgG. The sera of the patients were tested for anti‐liver‐specific lipoprotein by radioimmunoassay and for liver membrane autoantibody (by indirect immunofluorescence on isolated rabbit hepatocytes. The seven patients with normal liver or minor nonspecific alterations were negative for membrane IgG and serum antibodies.

Radioimmunoassay of Human Ligandin

Morris Sherman, Nathan M. Bass, John A. H. Campbell, Ralph E. Kirsch – 1 January 1983 – A sensitive and specific radioimmunoassay for human ligandin has been developed and used to study ligandin release into the serum in acute and chronic hepatitis. Serum ligandin concentrations were elevated in 67 of 68 cases of acute viral hepatitis. Ligandin levels frequently returned to normal within the first 2 weeks of illness. The rapid disappearance of ligandin preceded the return to normal of serum SGOT.

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