Hepatocyte Membrane‐Bound IgG and Circulating Liver‐Specific Autoantibodies in Chronic Liver Disease: Relation to Hepatitis B Virus Serum Markers and Liver Histology
Riccardo Meliconi, Federico Miglio, M. Valeria Stancari, Mario Baraldini, G. Francesco Stefanini, Giovanni Gasbarrini – 1 January 1983 – Hepatocytes isolated from 101 biopsies were examined for membrane‐bound IgG. The sera of the patients were tested for anti‐liver‐specific lipoprotein by radioimmunoassay and for liver membrane autoantibody (by indirect immunofluorescence on isolated rabbit hepatocytes. The seven patients with normal liver or minor nonspecific alterations were negative for membrane IgG and serum antibodies.
Radioimmunoassay of Human Ligandin
Morris Sherman, Nathan M. Bass, John A. H. Campbell, Ralph E. Kirsch – 1 January 1983 – A sensitive and specific radioimmunoassay for human ligandin has been developed and used to study ligandin release into the serum in acute and chronic hepatitis. Serum ligandin concentrations were elevated in 67 of 68 cases of acute viral hepatitis. Ligandin levels frequently returned to normal within the first 2 weeks of illness. The rapid disappearance of ligandin preceded the return to normal of serum SGOT.
Development and Use of a Rat Albumin cDNA Clone to Evaluate the Effect of Chronic Ethanol Administration on Hepatic Protein Synthesis
Mark A. Zern, Prasanta R. Chakraborty, Nelson Ruiz‐Opazo, Sing H. Yap, David A. Shafritz – 1 January 1983 – A rat albumin cDNA probe (pBR alb 149) was developed in order to investigate the molecular mechanisms responsible for changes in hepatic protein synthesis after chronic administration of ethanol to rats. Rats fed a diet for up to 1 year in which 36% of calories were from ethanol, developed fatty livers but not cirrhosis.
Measurement of Serum α1‐Acid Glycoprotein and cei‐Antitrypsin Desialylation in Liver Disease
Nathalie Serbource‐Goguel, Michèle Corbic, Serge Erlinger, Geneviève Durand, Jean Agneray, Jeanne Feger – 1 January 1983 – To determine whether the presence of circulating desialylated glycoproteins reflect the existence and/or the severity of liver disease, 73 patients were evaluated with liver biopsies, conventional liver function tests, and the measurement of the degree of desialylation of two glycoproteins ax‐acid glycoprotein (α1‐AGP) and ai‐antitrypsin (α1‐AT).
What is Actin Doing in the Liver Cell?
M. James Phillips, Chikara Oshio, Mamoru Miyairi, Sumio Watanabe, Charles R. Smith – 1 January 1983
Value of Screening for Markers of Hepatitis in Dialysis Units
Athol J. Ware, Nancy L. Gorder, Lawrence E. Gurian, Clark Douglas, James W. Shorey, Thomas Parker – 1 January 1983 – The value of monitoring the serum activity of SGOT, as well as markers of hepatitis B virus and hepatitis A virus infections, in the patients and staff of two dialysis units has been assessed retrospectively. Sera were checked each month for SGOT and HBsAg on 406 patients and 170 staff members over a 4‐year period. Anti‐HBc, anti‐HBs, and anti‐hepatitis A antibodies were assayed on the stored sera.
The Role of the Urea Cycle and Polyamines in Albumin Synthesis
Murray Oratz, Marcus A. Rothschild, Sidney S. Schreiber, Alvin Burks, Joseph Mongelli, Barbara Matarese – 1 January 1983 – Albumin synthesis is stimulated by those amino acids which increase urea synthesis and membrane bound polysome aggregation. Ornithine, an amino acid not incorporated into protein and produced from arginine in the urea cycle, is an albumin‐stimulating amino acid and is the precursor of the polyamines, and we have shown that the polyamine spermine promotes bound polysome aggregation.