Helmut Denk, Kurt Zatloukal, Karl‐Heinz Preisegger – 1 October 1989 – Polyclonal antibodies were raised which have a high affinity for conjugated ubiquitin. Immuno‐cytochemistry was performed on paraffin sections of tissues showing well‐characterized inclusion bodies. Ubiquitin was found as a component of the intermediate filament inclusion bodies characteristic of several major diseases including Lewy bodies of Parkinson's disease, Pick bodies of Pick's disease, Mallory bodies of alcoholic liver disease, cytoplasmic bodies of a specific myopathy, and Rosenthal fibres within astrocytes.

Russell H. Wiesner, Patricia M. Grambsch, E. Rolland Dickson, Jurgen Ludwig, Robert L. Maccarty, Ellen B. Hunter, Thomas R. Fleming, Lloyd D. Fisher, Sandra J. Beaver, Nicholas F. Larusso – 1 October 1989 – The natural history of primary sclerosing cholangitis was assessed in 174 patients; 37 were asymptomatic and 137 had symptoms related to underlying liver disease. At the time of diagnosis, the mean age was 39.9 years, 66% of the primary sclerosing cholangitis patients were male and 71% had associated inflammatory bowel disease, most commonly chronic ulcerative colitis.

Charles S. Lieber, Leonore M. Decarli, Michael F. Sorrell – 1 October 1989 – Techniques are reviewed for the experimental feeding of alcohol, including a liquid diet procedure invented 25 years ago. This technique results in much higher ethanol intake than with other approaches. As a consequence, various complications observed in alcoholics can be reproduced in animal models. These include fatty liver, hyperlipemia, various metabolic and endocrine disorders, tolerance to ethanol and other drugs, physical dependence and withdrawal and, in the baboon, liver fibrosis and cirrhosis.

David J. Mutimer, Shelley P. M. Fussey, Stephen J. Yeaman, Peter J. Kelly, Oliver F. W. James, Margaret F. Bassendine – 1 October 1989 – We have previously identified four of the M2 antigens in primary biliary cirrhosis as the E2 components (dihydrolipoamide acyltransferases) of pyruvate dehy‐drogenase complex, branched‐chain 2‐oxo acid dehy‐drogenase complex and 2‐oxoglutarate dehydrogenase complex and the protein X component of pyruvate dehydrogenase complex (approximate molecular masses: 74, 50, 50 and 52 kD, respectively).

Rachel Morecki, Joy Glaser – 1 October 1989 – Infection with reovirus 3 (Reo‐3) has been suggested as the cause of extrahepatic biliary atresia and idiopathic neonatal hepatitis but confirmation has been lacking. Therefore we have searched for a specific anti‐Reo‐3 antibody response in the sera of patients with biliary atresia or neonatal hepatitis and for Reo‐3 antigens in their hepatobiliary tissues.

Richard E. Sampliner, Albert J. Czaja, J. Eileen Hay, Jorge Rakela – 1 October 1989

1 October 1989

Yutaka Sasaki, Norio Hayashi, Yasuhiro Morita, Toshifumi Ito, Akinori Kasahara, Hideyuki Fusamoto, Nobuhiro Sato, Masaya Tohyama, Takenobu Kamada – 1 October 1989 – Expression of the c‐Ha‐ras proto‐oncogene is specifically enhanced during liver regeneration, in parallel with increased DNA replication, which suggests that c‐Ha‐ras may play a role in the control of regeneration. In this study, an in situ hybridization technique was applied for analysis of expression of the c‐Ha‐ras gene at the cellular level during liver regeneration induced by CCl4 administration.

Burton Combes – 1 October 1989

Mikihiro Tsutsumi, Jerome M. Lasker, Masanori Shimizu, Alan S. Rosman, Charles S. Lieber – 1 October 1989 – Perivenular hepatocytes are the first cells within the liver lobule to display signs of toxicity following long‐term alcohol use. In an attempt to explain this phenomenon, we have examined the hepatic intralobular distribution in rats and man of P450IIE1, a P‐450 isozyme that not only oxidizes ethanol but is also inducible by this agent.