A new three‐dimensional culture system for hepatocytes using reticulated polyurethane
Yasuo Sato, Takahiro Ochiya, Yuko Yasuda, Kenichi Matsubara – 1 April 1994 – Poly‐N‐para‐vinylbenzyl‐lactonamide (PVLA)‐coated reticulated polyurethane (PVLA‐RPU) has been employed for the long‐term maintenance of primary rat hepatocyte cultures. After 3 days of incubation of 2 × 107 hepatocytes/cm3 embedded in PVLA‐RPU discs and kept in culture medium, most cells showed typical hepatocyte morphology, with some bile canaliculus‐like intercellular spaces among the hepatocytes on examination with scanning and transmission electron microscopy.