Wan‐Long Chuang, Masao Omata, Toshiki Ehata, Osamu Yokosuka, Kazuhiko Hosoda, Fumio Imazeki, Masao Ohto – 1 March 1994 – The precore mutant hepatitis B virus often emerges from a mixed infection with combined wild‐type and precore mutant viruses. Nevertheless, the precore mutant does not seem to be an evolutionarily favored strain.
Deborah E. Sullivan, Michael A. Gerber – 1 March 1994 – Persistent infection by hepatitis C virus is a major risk factor for the development of hepatocellular carcinoma, but the mechanism of hepatocarcinogenesis is unknown. To study the association of hepatitis C virus with hepatocellular carcinoma, we sequenced part of the 5′ untranslated region of hepatitis C virus from the tumor tissue and the surrounding nontumorous liver of three patients with hepatocellular carcinoma. No sequence differences between tumor‐derived and liver‐derived hepatitis C virus isolates were detected.
Raymond S. Koff – 1 March 1994
William F. Balistreri – 1 March 1994 – “When medicine has really succeeded brilliantly in technology, as in immunization or endocrine‐replacement therapy, so that the therapeutic measures can be directed straight at the underlying disease mechanism and are decisively effective, the cost is likely to be very low indeed. It is when our technologies have to be applied halfway along against the progress of disease, or must be brought in after the fact to shore up the loss of destroyed tissue, that health care becomes enormously expensive.
Daniel P. O'Leary – 1 March 1994 – The more hydrophobic bile salts cause rapid release of preformed gallbladder mucin and other glycoproteins by gallbladder explants in vitro, whereas the less hydrophobic bile salts elicit a lesser response. This study was designed to determine (a) whether this short‐term effect was matched by a sustained increase in glycoprotein secretion over 24 hr and (b) whether it occurred when bile salts were presented in model biles rather than aqueous solution.
Jacquelyn J. Maher, Christina Tzagarakis – 1 March 1994 – Laminin is a heterotrimeric glycoprotein found in the perisinusoidal space of adult rat liver. The principal cellular source of laminin in liver is the lipocyte, with its three subunits measuring 324, 200 and 200 kD. The large subunit of lipocyte‐derived laminin is distinct from the A subunit of murine laminin (440 kD); its size suggests that it represents a peptide, called M, recently cloned from human placenta.
Rein M. J. Hoedemakers, Gerrit L. Scherphof, Toos Daemen – 1 March 1994 – We examined the effects of several hemopoietic growth factors on proliferation of rat liver macrophages in vitro. The proliferative response of liver macrophages to hemopoietic growth factors was assayed on the basis of [methyl‐3H]thymidine uptake. Macrophage colony‐stimulating factor and recombinant murine granulocyte‐macrophage colony‐stimulating factor stimulated [methyl‐3H]thymidine incorporation in a concentration‐dependent manner.
Sanjeev Gupta, Chang‐Don Lee, Ravikumar P. Vemuru, Kuldeep K. Bhargava – 1 March 1994 – Hepatocyte transplantation is useful for ex vivo gene therapy and liver repopulation. Methods for hepatic reconstitution have recently been developed but optimization of hepatocyte transplantation systems is necessary. To develop systems for noninvasive assessment of the biodistribution of transplanted cells, we labeled hepatocytes with 111indium‐oxine. Our initial studies showed that hepatocytes incorporated 111indium‐oxine with an efficiency of approximately 20%.
Massimo Bolognesi, Carlo Merkel, Simone Bianco, Paolo Angeli, David Sacerdoti, Piero Amodio, Angelo Gatta – 1 March 1994 – The reticuloendothelial system plays an important role in the prevention of bacterial infection in patients with cirrhosis. Few data are available, however, on its activity in such patients.
Joan C. García‐Pagán, Faust Feu, Antoni Castells, Angelo Luca, Ramón C. Hermida, Francisca Rivera, Jaume Bosch, Joan Rodés – 1 March 1994 – This study was aimed at investigating whether portal pressure, which is usually measured in the morning, is subject to circadian variations in patients with cirrhosis. Furthermore, a possible circadian variation for the occurrence of variceal bleeding was also investigated.