Matthias Blumrich, Renate Pack, Franz Oesch, Ernst Petzinger, Pablo Steinberg – 1 March 1994 – Freshly isolated oval cells, which we obtained from the livers of rats fed a choline‐deficient/DL‐ethioninesupplemented diet, did not transport bile acids. Compared with freshly isolated rat hepatocytes they took up only negligible amounts of [3H]taurocholate or [14C]cholate. The cells bound small amounts of radioactive bile acids. This portion of the total cell‐associated radioactivity was enhanced on membrane permeabilization.

Matthias Wettstein, Wolfgang Gerok, Dieter Häussinger – 1 March 1994 – We used the single‐pass–perfused rat liver model to study short‐term regulation of endotoxin‐inducible nitric oxide synthesis by following the release of nitrite and nitrate, the oxidation products of nitric oxide, into the effluent perfusate. In endotoxin‐pretreated livers, the basal nitrite + nitrate release was 5.3 ± 1.2 nmol·gm liver−1·min−1. Nitrite and nitrate release was stimulated by L‐arginine in a dose‐dependent and saturable fashion.

1 March 1994

Michele Maurice, Michael J. Schell, Bernard Lardeux, Ann L. Hubbard – 1 March 1994 – B10 is an integral glycoprotein of the plasma membrane that is exclusively localized to the canalicular (apical) domain in normal rat hepatocytes but may be expressed on the basolateral (sinusoidal and lateral) membrane in pathophysiological situations. To understand how B10 may be localized to the basolateral surface, we studied the biosynthesis and transport of this apical protein.

Maria Alfonsina Desiderio, Lidia Bardella – 1 March 1994 – Activity and messenger RNA levels of spermidine/spermine N1‐acetyltransferase, the rate‐limiting enzyme of the polyamine interconversion pathway, were investigated in host liver and in Yoshida AH‐130 ascites hepatoma cells as a function of tumor growth phases. Enzyme activity reached maximal values at day 10 in host liver (2.0‐fold increase) and at days 10 and 14 in hepatoma cells (4.2‐ and 5.4‐fold increases)– that is, when the cellular growth was nearly arrested.

Yochanan Peled, Tuvia Gilat – 1 March 1994 – The formation of cholesterol gallstones is a result of an interaction between bile lipid concentration, composition and other factors. The solubilization of cholesterol in bile is affected by the nature of the fatty acids and head groups of biliary phospholipids. In this study we tried to modulate the composition of biliary lipids in the rat and hamster by means of dietary supplementation of whole phospholipids or their constituents (at 3% to 5% of food weight).

Hiroshi Suemizu, Shinichi Yoshimura, Noritoshi Takeichi, Tetsuya Moriuchi – 1 March 1994 – The Long‐Evans Cinnamon rat is a mutant strain that contracts hereditary hepatitis and, eventually, spontaneous hepatoma. Recently, abnormal copper accumulations in Long‐Evans Cinnamon rat livers were shown to be genetically linked to the development of hepatitis.

Carole Oddoux, Gerd Grieninger – 1 March 1994 – This study characterizes plasma protein synthesis and its hormonal regulation in a chicken hepatoma cell line, with particular emphasis on fibrinogen. Whereas virtually all aspects of hemopexin, transferrin and albumin production in these cells corresponded to those of cultured primary hepatocytes, fibrinogen was not secreted. Analysis of fibrinogen subunit synthesis revealed a specific defect in synthesis of one subunit, γ, correlating with a lack of its mRNA.

David H. Adams, Patrizia Burra, Stefan G. Hubscher, Elwyn Elias, Walter Newman – 1 March 1994 – Alcoholic hepatitis is characterized by hepatocyte necrosis associated with infiltration of the liver parenchyma by neutrophils. The mechanisms responsible for recruiting neutrophils to the liver are unknown. We report high circulating levels and tissue expression of the endothelial adhesion molecule E‐selectin in alcoholic hepatitis.

Michael L. Schilsky, I. Herbert Scheinberg, Irmin Sternlieb – 1 March 1994 – The objective of this study was to determine the indications for and results of liver transplantation in patients with Wilson's disease on the basis of results of a survey with retrospective review of data obtained on 55 transplants performed at centers in the United States and Europe. The study group comprised 32 females and 23 males, aged 8.5 to 51 yr, with features diagnostic of Wilson's disease.