Immunoelectron microscopic observation of intrahepatic HBeAg in patients with chronic hepatitis B
Gotaro Yamada, Kouichi Takaguchi, Kazuhiro Matsueda, Hiroshi Nishimoto, Michiko Takahashi, Shigeatsu Fujiki, Motowo Mizuno, Shingo Kinoyama, Takao Tsuji – 1 July 1990 – Immune light and electron microscopic studies using monoclonal antibodies have been applied to localize HBeAg in liver biopsy specimens of 19 patients with chronic hepatitis B. Under the light microscope, HBeAg was demonstrated in nuclei, cytoplasm and on the cell surface of hepatocytes. The number of HBeAg‐positive hepatocytes correlated well with the serum levels of HBeAg (enzyme immunoassay) and DNA‐polymerase.