Alterations in nuclear scaffold constituents during carbon tetrachloride–induced liver regeneration

Gary A. Clawson, Kenneth R. Madsen, Lori J. Blankenship, Christine L. Hatem – 1 March 1991 – Liver regeneration was induced in rats by treatment with CCl4, which results in substantial regenerative activity with a sharp mitotic response 2 days after intoxication. Closely paralleling the mitotic index, we observed fourfold increases in nuclear scaffold nucleoside triphosphatase, an activity thought to participate in nucleocytoplasmic RNA transport and in the 46 kD putative enzyme and its selective photolabeling.

Ethanol increases the formation of NADP+ in rat hepatocytes

B. Emmanuel Akinshola, James J. Potter, Esteban Mezey – 1 March 1991 – The acute effects of ethanol on total (bound + free) pyridine dinucleotides were determined in freshly isolated rat hepatocytes. Pyridine dinucleotides and adenine nucleotides were determined by highperformance liquid chromatography. Exposure of the hepatocytes to 8 mmol/L ethanol resulted in a decrease in NAD+ and an increase in NADP+ after 2 min incubation. There were no significant changes in NADH and NADPH.

Hypothermic preservation of hepatocytes. III. Effects of resuspension media on viability after up to 7 days of storage

Diane C. Marsh, Julie A. Hjelmhaug, Paul K. Vreugdenhil, Julie A. Kerr, Mark J. Rice, Folkert O. Belzer, James H. Southard – 1 March 1991 – Hepatocyte suspensions provide a rapid method to determine how hypothermic storage affects liver cell metabolism and viability. Using these studies, improved methods of hypothermic liver preservation for transplantation may be developed. In this study, rat hepatocytes were cold‐stored for up to 7 days in University of Wisconsin liver preservation solution.

Usefulness of pulsed doppler ultrasound in detection of angiographically evident recurrence of hepatocellular carcinoma after arterial embolization treatment

Satoshi Mochida, Shigeki Hayashi, Itsuro Ogata, Naohiko Masaki, Sumiko Nagoshi, Tomoaki Tomiya, Akihiko Ohno, Katsuyoshi Takatsuki, Yasuhiko Ohta, Shinwa Yamada, Yuzuru Sato, Kuni Ohtomo, Kenji Fujiwara – 1 March 1991 – Because hepatocellular carcinoma treated by transcatheter arterial embolization often regains its size, routine follow‐up is necessary. The usefulness of pulsed Doppler ultrasound for detection of this type of recurrence was compared with ultrasonography and computed tomography in 21 such hepatocellular carcinomas.

A new method to monitor Kupffer cell phagocytosis continuously in perfused rat liver

Patricia E. Ganey, Barbara Keller, Steven N. Lichtman, John J. Lemasters, Ronald G. Thurman – 1 March 1991 – A new method was developed to monitor Kupffer cell phagocytosis continuously in perfused liver using a fluorescent probe, rhodamine‐gelatin, synthesized from gelatin and rhodamine isothiocyanate. In perfused rat liver, phagocytosis by Kupffer cells was assessed both by uptake of the dye and from fluorescence measured from the liver surface. Uptake of rhodamine‐gelatin and surface fluorescence (520 → 585 nm) increased as perfusate concentrations of rhodamine‐gelatin were elevated.

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