Steven N. Lichtman, John Keku, Richard L. Clark, John H. Schwab, Ryan B. Sartor – 1 April 1991 – Small bowel bacterial overgrowth, which develops in surgically created jejunal self‐filling blind loops, is associated with hepatic injury in susceptible rat strains. The histological findings are portal tract inflammation and bile duct proliferation and destruction.
Kenneth R. Feingold, Mary E. Barker, Albert L. Jones, Carl Grunfeld – 1 April 1991 – Tumor necrosis factor and other cytokines mediate the body's response to infection and inflammation. Long‐term administration of tumor necrosis factor causes liver hypertrophy, and our laboratory has shown that tumor necrosis factor acutely increases hepatic DNA synthesis. The purpose of this study was to determine which specific cell types in the liver undergo DNA synthesis in response to tumor necrosis factor.
Alastair J. Macgilchrist, David Sumner, John L. Reid – 1 April 1991 – The blood pressure responses to intravenous infusions of norepinephrine and angiotensin II, sympathetic and nonsympathetic vasoconstricting agents, respectively, were measured in 20 patients with cirrhosis (10 Child‐Pugh grade A and 10 Child‐Pugh grades B or C) and in 20 healthy subjects. The log PD20 (dose of agonist required to raise blood pressure by 20 mm Hg) for norepinephrine was 4.78 ± 0.36 (mean ± S.D.) in patients with severe cirrhosis and 4.36 ± 0.37 in controls, p < 0.01.
Peter T. Donaldson, Derek G. Doherty, Karen M. Hayllar, Ian G. McFarlane, Philip J. Johnson, Roger Williams – 1 April 1991 – After nearly 18 years of research, the association between human leukocyte antigens A1‐B8‐DR3 and autoimmune chronic active hepatitis still provokes debate. The principal reasons for this are disease heterogeneity and racial variation in the distribution of human leukocyte antigens between populations.
Jorge J. Gumucio, Michael C. Kew – 1 April 1991 – To determine serum thyroxine‐binding globulin (TBG) levels, we used radioimmunoassay, and compared the results obtained with other tests in 231 patients with chronic hepatitis B virus infection to evaluate its clinical implications. All of these patients were hepatitis B surface antigen (HBsAg)‐positive.
Patricia E. Ganey, Barbara Keller, Steven N. Lichtman, John J. Lemasters, Ronald G. Thurman – 1 March 1991 – A new method was developed to monitor Kupffer cell phagocytosis continuously in perfused liver using a fluorescent probe, rhodamine‐gelatin, synthesized from gelatin and rhodamine isothiocyanate. In perfused rat liver, phagocytosis by Kupffer cells was assessed both by uptake of the dye and from fluorescence measured from the liver surface. Uptake of rhodamine‐gelatin and surface fluorescence (520 → 585 nm) increased as perfusate concentrations of rhodamine‐gelatin were elevated.
1 March 1991
Satoshi Mochida, Shigeki Hayashi, Itsuro Ogata, Naohiko Masaki, Sumiko Nagoshi, Tomoaki Tomiya, Akihiko Ohno, Katsuyoshi Takatsuki, Yasuhiko Ohta, Shinwa Yamada, Yuzuru Sato, Kuni Ohtomo, Kenji Fujiwara – 1 March 1991 – Because hepatocellular carcinoma treated by transcatheter arterial embolization often regains its size, routine follow‐up is necessary. The usefulness of pulsed Doppler ultrasound for detection of this type of recurrence was compared with ultrasonography and computed tomography in 21 such hepatocellular carcinomas.
Diane C. Marsh, Julie A. Hjelmhaug, Paul K. Vreugdenhil, Julie A. Kerr, Mark J. Rice, Folkert O. Belzer, James H. Southard – 1 March 1991 – Hepatocyte suspensions provide a rapid method to determine how hypothermic storage affects liver cell metabolism and viability. Using these studies, improved methods of hypothermic liver preservation for transplantation may be developed. In this study, rat hepatocytes were cold‐stored for up to 7 days in University of Wisconsin liver preservation solution.
B. Emmanuel Akinshola, James J. Potter, Esteban Mezey – 1 March 1991 – The acute effects of ethanol on total (bound + free) pyridine dinucleotides were determined in freshly isolated rat hepatocytes. Pyridine dinucleotides and adenine nucleotides were determined by highperformance liquid chromatography. Exposure of the hepatocytes to 8 mmol/L ethanol resulted in a decrease in NAD+ and an increase in NADP+ after 2 min incubation. There were no significant changes in NADH and NADPH.