Conditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin‐UDP‐glucuronosyltransferase gene transfer
Ira J. Fox, Namita Roy Chowdhury, Sanjeev Gupta, Ravi Kondapalli, Michael L. Schilsky, Richard J. Stockert, Jayanta Roy Chowdhury – 1 March 1995 – Viral vectors and protein carriers utilizing asialoglycoprotein receptor (ASGR)‐mediated endocytosis are being developed to transfer genes for the correction of bilirubin‐UDP‐glucuronosyltransferase (bilirubin‐UGT) deficiency. Ex vivo evaluation of these gene transfer vectors would be facilitated by a cell system that lacks bilirubin‐UGT, but expresses differentiated liver functions, including ASGR.