Poul Schlichting, Erik Christensen, Per K. Andersen, Lis Fauerholdt, Erik Juhl, Hemming Poulsen, Niels Tygstrup, THE COPENHAGEN STUDY GROUP FOR LIVER DISEASES – 1 January 1983 – In a controlled clinical trial in 488 patients with chronic liver disease treated with prednisone or placebo, survival data were analyzed using Cox's proportional hazards model. A total of 162 variables were screened separately for prognostic and/or therapeutic effect by log‐rank analyses, whereby 46 clinical, biochemical, serological, and histological variables were isolated.

Scheffer C. G. Tseng, Edward A. Smuckler, Robert Stern – 1 January 1983 – Collagen formation is an important function of liver parenchymal cells that may be relevant to the pathogenesis of hepatic fibrosis. The types of collagen synthesized by cultured normal rat liver hepatocytes were examined. Cells isolated from adult rats by enzymatic dispersion of the liver were established in primary monolayer culture. Cells were then incubated with radiolabeled proline for 20 hr in the presence of ascorbate and the lathrogen β‐aminopropionitrile.

Gary A. Levy, Peggy J. Macphee, Lai Sum Fung, Murray M. Fisher, Aron M. Rappaport – 1 January 1983 – Mouse hepatitis virus type 3 infection results in strain‐dependent liver disease. The effects of mouse hepatitis virus type 3 on the microcirculation of the liver in both fully susceptible (Balb/cJ) and fully resistant (A/J) mice were studied. In Balb/cJ mice, 6 to 12 hr following infection, abnormalities in liver blood flow were observed which consisted of granular blood flow in both terminal hepatic and terminal portal venules.

Janet D. Elashoff – 1 January 1983

Jacques Scotto, Michelle Hadchouel, Christiane Hery, Jeannine Yvart, Pierre Tiollais, Christian Brechot – 1 January 1983 – A simplified spot method for determination in serum of hepatitis B virus DNA (HBV DNA) by molecular hybridization is proposed. For simultaneous testing of 30 serum samples, it reduced to about 1 hr the duration of the steps preceding hybridization proper. The method also greatly reduced the loss of DNA during these steps and allowed more sensitive detection in samples of only 25 or 50 m̈1.

Harvey M. Lieberman, Douglas R. Labrecque, Michael C. Kew, Stefanos J. Hadziyannis, David A. Shafritz – 1 January 1983 – A simple, direct molecular hybridization test was employed to detect hepatitis B virus (HBV) DNA sequences in human serum. In 61 HBsAg carriers, many with HBV‐related diseases (chronic persistent hepatitis, chronic active hepatitis, or posthepatitic cirrhosis), 28/28 (100%) who were HBeAg* and 16/32 (50%) who were anti‐HBe+ had HBV DNA sequences in their serum.

Shaindel Y. Mishkin, Emmanuel Farber, Ru Kun Ho, Shree Mulay, Seymour Mishkin – 1 January 1983 – Hepatic hyperplastic nodules (HHNs) in rats were studied as an experimental prototype of oral contraceptive‐related hepatic tumors. We have found cytoplasmic estrogen receptors in HHNs produced by acetylaminofluorene (AAF) (four cycles of 0.02% in diet). Rats with AAF‐induced HHNs were randomized into four groups: (i) AAF‐treated control; (ii) estrogen alone (estradiol‐17β); (iii) tamoxifen alone, and (iv) estrogen + tamoxifen.

Robert Barouki, Marie‐Noële Chobert, Joëlle Finidori, Martine Aggerbeck, Bertrand Nalpas, Jacques Hanoune – 1 January 1983 – The clone C2 derived from a rat hepatoma cell line was used to investigate the mechanism of the induction of γ‐glutamyltransferase by ethanol. γ‐glutamyltransferase activity was detected in the C2 cell (1.4 mU per mg protein), and its kinetic properties were similar to normal rat liver γ‐glutamyltransferase.

Fernando Alvarez, Pierre Landrieu, Paul Laget, Frédérique Lemonnier, Michel Odièvre, Daniel Alagille – 1 January 1983 – Thirteen children, aged 10 months to 20 years, presenting with chronic cholestasis from the first month of life and with low serum levels of vitamins A and/or E, have been investigated for neurological and ophthalmological symptoms. Clinical findings consisted of 4 types: peripheral neuropathy; cerebellar dysfunction; abnormalities of eye movement, and retinal degenerative changes.

Urs A. Boelsterli, Gopa Rakhit, Tibor Balazs – 1 January 1983 – Structural and functional changes in the surface membranes of hepatocytes play a pivotal role in the induction and reversion of some forms of drug‐induced cholestasis. To elucidate the mechanism by which S‐adenosyl‐L‐methionine (SAMe) leads to a partial reversion of bile flow impairment caused by ethinyl estradiol (EE), female Sprague‐Dawley rats were given oral doses of EE (5 mg per kg per day, for 3 days) with and without simultaneous administration of SAMe (25 mg per kg, 3 times per day, for 3 days).