J. Loren Pitcher – 1 September 1987

Keith D. Lindor, Russell H. Wiesner, Nicholas F. Larusso, Henry A. Homburger – 1 September 1987 – Primary sclerosing cholangitis is a chronic, cholestatic liver disease in which immune mechanisms are thought to play a role in the pathogenesis. We have determined the frequencies and functional phenotypes of autoreactive T‐lymphocytes in peripheral blood specimens from patients with primary sclerosing cholangitis and healthy adults as an indicator of autoreactivity in primary sclerosing cholangitis.

1 September 1987

1 September 1987

H. C. Thomas, S. A. Jenkins, J. N. Baxter, R. Shields – 1 September 1987

Thomas H. Maren – 1 September 1987 – 1. In isolated perfused rat liver, urea synthesis is rapid and reversibly inhibited not only by the well‐known carbonic anhydrase inhibitors acetazolamide, methazolamide and ethoxzolamide, but also by diuretics, like xipamide, mefruside, chlortalidone, and chlorothiazide. Furosemide was without effect.

Gang‐Hong Lee, Tokuichi Kawaguchi, Kimie Nomura, Tomoyuki Kitagawa – 1 September 1987 – Woodchucks free from woodchuck hepatitis virus were treated with diethylnitrosamine in vivo for 2 months, and then hepatocytes obtained by enzymatic perfusion were cultured with the hepatopromoter phenobarbital. This in vivo‐in vitro procedure gave rise to proliferating epithelial cell foci, from one of which the presently described hepatocyte cell line (WLC‐3) was established and characterized.

Antonio Francavilla, Lorenzo Polimeno, David H. Van Thiel, Saturo Todo, Igal Kam, Stephen Lynch, Thomas E. Starzl – 1 September 1987 – Glucose intolerance, hyperinsulinemia, peripheral insulin resistance and hyperglucagonemia are common in patients with advanced liver disease. These abnormalities in the plasma levels of the pancreatic hormones, insulin and glucagon have been thought to be responsible, at least in part, for the abnormal plasma ratio of branched‐chain amino acids to aromatic amino acids.

Richard A. Robbins, Rowen K. Zetterman, Todd J. Kendall, Gail L. Gossman, Howard P. Monsour, Stephen I. Rennard – 1 September 1987 – Defective regulation of neutrophil chemotaxis occurs in patients with alcoholic liver disease. One potent mediator of neutrophil chemotaxis is the complement‐derived neutrophil chemoattractant, C5a, which can be inhibited by a serum protein, chemotactic factor inactivator. We hypothesized that chemotactic factor inactivator elevation might, in part, explain the defective neutrophil chemotaxis seen in patients with alcoholic hepatitis.

Hartmut Rieder, Giuliano Ramadori, Hans‐Peter Dienes, Karl‐Hermann Meyer Zum Büschenfelde – 1 September 1987 – Endothelial liver cells were obtained from guinea pig by enzymatic digestion and centrifugal elutriation. Cells were cultured on gelatin and fibronectin pretreated culture vessels. Endothelial cells were characterized by phase‐contrast microscopy, electron microscopy and the presence of Factor VIII‐related antigen. Fibronectin secretion was determined in cell‐free supernatants by a sensitive and specific ELISA and localized on fixed cultured cells by immunofluorescence.