Aldo Roda, Adamo Fini – 1 September 1984 – The aqueous solubility and thermodynamic dissociation constants of a representative series of bile acids with varying numbers and configuration of nuclear hydroxyl substituents were determined. The pKa values were calculated by extrapolating pKa' values determined in solutions of aqueous methanol of different mole fractions at 25o C.
Yves Deugnier, Véronique David, Pierre Brissot, Philippe Mabo, Damien Delamaire, Michel Messner, Michel Bourel, Jean‐Yves Legall – 1 September 1984 – α‐L‐fucosidase, a lysosomal enzyme which catabolizes fucoproteins, was studied in sera from 30 controls, 32 patients with primary hepatic carcinomas, 24 patients with secondary metastatic liver carcinomas and 36 patients with cirrhosis.
Alan M. Leichtner, Monty Krieger, Alan L. Schwartz – 1 September 1984 – Low density lipoprotein (LDL) processing was investigated in a human hepatoma‐derived cell line, Hep G2. Hep G2 cells bound, internalized and degraded LDL via a saturable, high affinity (Kd — 2 ± 10−8M) pathway similar to that present in other mammalian cells. Although 80% of the uptake and degradation of 125I‐LDL was inhibited by 40‐fold excess native LDL, the same concentration of methylated LDL, which cannot bind to LDL receptors, had virtually no effect on processing.
Bruce A. Runyon, John C. Hoefs, Carmen Viola, Lydia Viñeta, Jaime Bosch, Juan Rodés – 1 September 1984
Josip P. Kratohvil – 1 September 1984 – The current state of the knowledge of the size and the aggregation numbers of micelles formed in solutions of bile salts was evaluated. The experimental techniques considered include static light scattering, sedimentation equilibrium, membrane osmometry, sedimentation velocity and transla‐tional diffusion. The theoretical background of each method is briefly discussed, the working equations are summarized, and the limitations of each technique are enumerated.
Peter Schurtenberger, Norman A. Mazer, Werner Känzig – 1 September 1984 – Using quasielastic light scattering, we investigated the formation of vesicles produced spontaneously by diluting a mixed micellar solution of bile salt and lecithin beyond the micellar phase boundary. The vesicles were nearly monodisperse, and their size varied between 120 to 500 A depending upon the dilution conditions. A systematic study of the dependence of the vesicle size upon the bile salt and lecithin concentration was made and interpreted in terms of a simple equilibrium model.
Stephen Barnes – 1 September 1984 – Studies of bile salt structure using nuclear magnetic resonance (NMR) and X‐ray crystallography have suggested that micellar association of bile salts is a function of side chain length and of packing of the side chain and the steroid nucleus. Lanthanide NMR probe analysis has been used to determine the structure of what is probably the monomer of the bile salt glycocholate.
Siegfried Lindenbaum, Natarajan Rajagopalan – 1 September 1984 – The kinetics of dissolution of dispersions of egg phosphatidylcholine (lecithin) by bile salts was studied by observing the decrease in turbidity as mixed micelles of lecithin and bile salts were formed. The rate of dissolution of lecithin corresponding to formation of mixed micelles was studied in the presence of dihydroxy bile salts, sodium deoxycholate, sodium chenodeoxycholate, sodium ursodeoxycholate and one trihydroxy bile salt, sodium cholate.
Martin C. Carey – 1 September 1984 – Cholesterol is solubilized by bile salt and bile salt‐lecithin micelles without appreciable alteration in preexisting micellar size or structure. In dilute (∼ 3 gm per dl) model bile, but not in concentrated (∼ 10 gm per dl) systems, supramicellar concentrations of cholesterol are also solubilized in “stable” microdispersions of small lecithin‐cholesterol vesicles (∼ 200 to 400 A in radius).
R. Thomas Holzbach – 1 September 1984 – Major discrepancies exist between both the degree (or cholesterol excess limit) and duration of metastable cholesterol supersaturation in concentrated model biles and native human gallbladder bile. The comparatively reduced metastability of in vitro model systems suggests that a stabilizing factor is absent in these more concentrated systems. In contrast, the metastable supersaturation behavior of the dilute model and hepatic biles is similar particularly with respect to their markedly prolonged metastability, i.e., nucleation time.