Francois Ballet, Marie‐Elisabeth Bouma, Shu‐Ren Wang, Norma Amit, Jacqueline Marais, Recaredo Infante – 1 September 1984 – A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 ± 107 cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characteristics were similar to hepatocytes in vivo. Bile canaliculi were observed by electron microscopy.

H. Theo M. Cuypers, El Terlen M. Haar, Peter L. M. Jansen – 1 September 1984 – Bilirubin monoglucuronide is rapidly deconjugated when incubated with UDP and rat liver microsomal preparations at pH 5.1.

W. Wayne Lautt, Dallas J. Legare, Timothy R. Daniels – 1 September 1984 – Compounds reaching the liver do so via either the hepatic artery or the portal vein. This paper reports on the effectiveness of administration of compounds into these alternate routes for their effects on the hepatic parenchymal cells, the hepatic arterial resistance vessels (blood flow) and hepatic capacitance (blood volume responses). All tests were done on cats under pentobarbital anesthesia.

Adrian Reuben – 1 September 1984

Adrian Reuben – 1 September 1984 – The study of biliary proteins has grown enormously in the last 10 years. Although much has been recently learned about the nature, origin and hepatobiliary transport of these proteins, little is known of their function in bile or their effect on its physical state. This review will focus on description of the proteins and mechanisms by which they are secreted into bile.

1 September 1984

Alan F. Hofmann – 1 September 1984 – Bile contains four calcium anions which are readily precipitated by calcium: bilirubinate, “palmitate”, carbonate and phosphate. Precipitation of insoluble calcium salts is common in calculous biliary disease. All animal models of calcium cholelithiasis probably share a common pathogenesis‐an increased concentration of a calcium‐sensitive anion. In calcium bilirubinate cholelithiasis, an increased concentration of bilirubinate occurs.

Johannes Gaub, Johan Iversen – 1 September 1984 – In previous studies, 90% partial hepatectomy in the rat was invariably accompanied by 100% mortality within 40 hr. This paper describes a technique by which 90% of the liver mass can be removed with only 14% mortality, provided that rats have free access to glucose‐containing drinking water. Measurements of total liver DNA, [3H]thymidine labeling index and mitotic index suggest rapid cell proliferation, commensurate with a powerful regenerative stimulus.

Lisa Lo Presti – 1 September 1984

Steven M. Strasberg, P. Robert C. Harvey, Steven Gallinger – 1 September 1984 – Difficulties in obtaining proper bile samples are due to inaccessibility of the biliary tree and to distortions induced by sampling methods. One must be cognizant of the effects of diet on bile secretion and gallbladder motility. Experimental methods which interrupt the enterohepatic circulation or alter the intestinal migrating myoelectric complex induce spurious changes in bile flow and composition. Biliary tract pressure‐flow relationships must be maintained or the gallbladder will be made functionless.