Hubertus Nederbragt, Aart J. Lagerwerf – 1 July 1986 – Biliary copper excretion was studied in male, bilecannulated rats of the inbred strains Fischer, Brown Norway, WAG/Rij and Lewis. After intravenous injection of 10, 30 and 50 μg copper per 100 gm body weight, two patterns of copper excretion were observed; their profiles varied with the copper dose and the strain of the rats used. The lowest amounts of copper were excreted by Fischer rats, the highest by WAG/Rij rats; this was related to the effect of the copper dose on both patterns.

Jerome B. Zeldis – 1 July 1986 – Nucleocapsid‐pol fusion proteins have been detected by serological screening hepatocellular carcinoma tissues that contain hepatitis B virus (HBV) DNA. The existence of these fusion proteins suggests that HBV may synthesize its reverse transcriptase in a fashion analogous to the way that retroviruses synthesize and process a precursor. The accumulation of HBV reverse transcriptase intermediates in tumorous tissues and not in other tissues may be related to the absence of viral core particles and possibly contributes to tumor development.

Lawrence Lumeng – 1 July 1986

Yasuni Nakanuma, Goroku Ohta, Hitoshi Sugiura, Kishichiro Watanabe, Kenji Doishita – 1 July 1986 – The morphologic features and growth pattern of single hepatocellular carcinomas ⩽ 1 cm in size, found incidentally at autopsy, were studied in nine cases. In all but one case, the hepatic parenchyma showed advanced cirrhosis. In three cases, the hepatocellular carcinomas were localized within a regenerative nodule as a form of “nodule within nodule.” The carcinoma was rimmed by nonneoplastic hepatic tissue.

Thomas M. Kloppel, William R. Brown, Juerg Reichen – 1 July 1986 – Employing the in situ perfused rat liver, we examined the origins and mechanisms of transport of proteins into bile. First, utilizing polyacrylamide gels, we noted that many biliary proteins co‐migrated with dominant serum proteins. Upon liver perfusion with serum‐free medium, most proteins disappeared from the biliary profile; one major biliary protein that was not present in serum, identified as secretory component, remained.

1 July 1986

Costantino de Giacomo, Maria S. Scotta, Silvia Perversi, Gian Vico M. Melzi D'Eril, Giuseppe Maggiore – 1 July 1986

John P. Cello, David Schneiderman – 1 July 1986

Luc Bouwens, Marijke Baekeland, Ronald de Zanger, Eddie Wisse – 1 July 1986 – In normal rat liver, Kupffer cells were unequivocally identified using peroxidase cytochemistry by light microscopy in semithin plastic sections. The Kupffer cell population was found to constitute 31% of the sinusoidal cells and by morphometry and serial sectioning, a mean absolute number of 14 to 20 × 106 Kupffer cells per g liver was calculated. The mean distribution of Kupffer cells in the liver lobules was 43% in the periportal, 28% in the midzonal and 29% in the central area of the lobule.

Michel Charbonneau, Beatriz Tuchweber, Gabriel L. Plaa – 1 July 1986 – The ability of ketonic compounds to modify the chronic liver injury (cirrhosis) induced by haloalkanes is unknown. To investigate this problem, male Sprague‐Dawley rats were treated p.o. twice weekly for 12 weeks with acetone (25 mmoles per kg in corn oil) or corn oil alone (10 ml per kg). The rats were treated p.o. with corn oil (10 ml per kg) or CCl4 (5 mmoles per kg in corn oil) 18 hr after each pretreatment. Animals were killed after 4, 8, 10 or 12 weeks of treatment.