Rat hepatic bile acid sulfotransferase: Identification of the catalytic polypeptide and evidence for polymeric forms in female rats
Robert H. Collins, Leon Lack, Kenneth M. Harman, Paul G. Killenberg – 1 July 1986 – A monoclonal antibody, PK1B, directed against rat liver bile acid sulfotransferase was used for the purification and characterization of the enzyme. Incubation of rat liver supernatant with the antibody followed by immunoprecipitation with Staphylococcus aureus cells demonstrated that PK1B reacted with 90% of the enzymatic activity present in the liver supernatant from female rats and 40 to 50% of the activity in male liver preparations.