Interaction of natural and synthetic albumin polymers with hepatocytes

Teresa L. Wright, Nina Lysenko, Robert K. Ockner, Richard A. Weisiger – 1 March 1987 – The hepatitis B virus binds avidly to albumin polymers which in turn may mediate the initial binding of viral particles to the liver cell. However, the interaction of albumin polymers with the liver remains poorly characterized, and the possibility that hepatic binding reflects an artifact of polymerization with glutaraldehyde has not been excluded. We therefore characterized the binding of 125I‐labeled natural and synthetic albumin polymers to suspensions of rat hepatocytes.

An extrahepatic bile duct growth factor: In vivo effect and preliminary characterization

Joy H. Glaser, Rachel Morecki, Sheila Fallon‐Friedlander, Marshall S. Horwitz – 1 March 1987 – Rabbit sera injected intraperitoneally into BALB/c mice were noted to produce a considerable and selective enlargement of extrahepatic bile ducts. The gallbladder, intrahepatic ducts, liver and other organs showed no stimulaion of growth. Duct enlargement leading to widening of its outer diameter which, on average, was 3.6 times that of normal, was due entirely to an increased number of epithelial cells with prominent proliferation of intramural glandular components.

Inflammatory pseudotumor of the liver: An enigmatic lesion with polyclonal plasma cell proliferation

Eric B. Larson – 1 March 1987 – Five cases of inflammatory pseudotumour of the liver are reported and compared with seven individual previously published case reports. Clinical presentation was variable but often comprised low grade intermittent fever, vague abdominal symptoms, and a history of weight loss. Leucocytosis, raised erythrocyte sedimentation rate, and polyclonal hyperglobulinaemia were also sometimes detected. All five cases in the present series were considered to be clinically malignant; and in two the histological diagnosis was also that of malignancy.

Hepatic metabolism during acute ethanol administration: A phosphorus‐31 nuclear magnetic resonance study on the perfused rat liver under normoxic or hypoxic conditions

Franck Desmoulin, Paul Canioni, Christian Crotte, André Gérolami, Patrick J. Cozzone – 1 March 1987 – The effect of ethanol metabolism on the energetic parameters and intracellular pH of the isolated perfused rat liver from fed rats was studied by phosphorus‐31 nuclear magnetic resonance spectroscopy. This technique allowed us to analyze nondestructively and in real time the role of low oxygen tension on the possible injurious effect of ethanol on the liver cells.

Characterization of desmin‐positive rat liver sinusoidal cells

Mikihiro Tsutsumi, Akira Takada, Shujiro Takase – 1 March 1987 – Hepatic sinusoidal lining cells were isolated from mature male rats (12 months old) and cultured in order to clarify the characteristics of the Ito cells (fat‐storing cells). Desmin staining was used as a marker in the isolation of the Ito cells, and a highly purified desmin‐positive cell fraction was obtained. Less than 10% of the desmin‐positive cells were positive for vitamin A auto‐fluorescence.

Bile acid transport: Lessons from the intestine

Richard H. Moseley – 1 March 1987 – The transport of bile acid was studied in basolateral membrane vesicles isolated from rat small intestine. Taurocholate transport into an osmotically reactive intravesicular space was Na+ independent. The uptake of taurocholate in jejunal and ileal vesicles preloaded with sulfate was stimulated with respect to uptake in unpreloaded vesicles. Glycocholate inhibited the transstimulation of taurocholate uptake by sulfate.

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