George Zahrebelski, Anna‐Liisa Nieminen, Kristin Al‐Ghoul, Ting Qian, Brian Herman, John J. Lemasters – 1 May 1995 – The aim of this study was to evaluate changes in the subcellular organelles of cultured hepatocytes by laser scanning confocal microscopy during chemical hypoxia with cyanide and iodoacetate, inhibitors of mitochon‐drial respiration and glycolysis, respectively.

Juan Cordoba, Andres T. Blei – 1 May 1995 – With the wide acceptance of liver transplantation as a therapeutic alternative in fulminant hepatic failure (FHF), the successful management of patients with this syndrome has acquired a new urgency. Topping the list of medical problems is the development of brain swelling. Two decades after the recognition of its importance,1 brain edema and intracranial hypertension still constitute a major cause of death in these patients.

Tatu A. Miettinend, Martti Färkkilä, Matti Vuoristo, Anna‐Liisa Karvonen, Rauli Leino, Juhani Lehtola, Claes Friman, Kari Seppälä, Juhani Tuominen – 1 May 1995 – A randomized placebo‐controlled 2‐year study was performed in 69 patients with primary biliary cirrhosis (PBC) on serum lipids during ursodeoxycholic acid (URSO) and colchicine treatments.

Romana Lenzen, Peter Stark, Viktoria Kolb‐Bachofen, Georg Strohmeyer – 1 May 1995 – In guinea pigs, glucagon choleresis is accompanied by a significant, but transient, stimulation of biliary protein secretion, which can be accounted for mainly by biliary discharge of lysosomal enzymes.

Pieter J. De Bleserc, Peggy Jannes, Sylvia C. Van Buul‐Offers, Cok M. Hoogerbrugge, Christiaan F. H. Van Schravendijk, Toshiro Niki, Vera Rogiers, Jan L. Van Den Brande, Eddie Wisse, Albert Geerts – 1 May 1995 – Transforming growth factor β (TGF‐β), a potent fibrogenic cytokine, is secreted in latent form. We examined which cell type in both normal and carbon tetrachloride (CCl4)‐induced fibrotic rat liver bears surface type II IGF/mannose 6‐phosphate (IGF‐II/M6P) receptor, known to facilitate activation of TGF‐β.

Koichi Urata, Seiji Kawasaki, Hidetoshi Matsunami, Yasuhiko Hashikura, Toshihiko Ikegami, Shinpachi Ishizone, Yoshitaka Momose, Atsushi Komiyama, Masatoshi Makuuchi – 1 May 1995 – Despite refinements in surgical techniques for liver transplantation, liver size disparity remains one of the most common problems in pediatric patients. Optimal liver graft size remains unknown and the volume of diseased liver in the recipient is not indicative of the volume (standard liver volume [LV]) optimal for the recipient's metabolic demands.

Humberto Reyes, José Ribalta, Ismael Hernández, Marco Arrese, Nelly Pak, Michael Wells, Ralph E. Kirsch – 1 May 1995 – The hypothesis that dietary erucic acid may contribute to the pathogenesis of intrahepatic cholestasis of pregnancy has been examined in pregnant rats and hamsters after prolonged feeding of diets containing 25% rapeseed oil rich in erucic acid (40% of fatty acids) or corn oil, without erucic acid. Both dietary oils were well tolerated, although weight gain was 17% to 20% less in unimals receiving rapeseed oil.

Hirokazu Yokoyama, Shigeyuki Nagata, Susumu Moriya, Shinzo Kato, Takashi Ito, Kiyotaka Kamegaya, Hiromasa Ishii – 1 May 1995 – Experimental hepatic fibrosis was produced in the guinea pig. We produced hepatic necrosis associated with inflammatory cell infiltration in guinea pigs immunized with acetaldehyde adducts and fed ethanol for 40 days. Extending the period of these treatments to 90 days resulted in producing hepatic fibrosis developing around individual hepatocytes in the terminal hepatic venule areas and portal areas, accompanied by an increase in hepatic hydroxyproline content.

Gianluigi Vendemiale, Ferruccio Guerrieri, Ignazio Grattagliano, Domenico Didonna, Leonilde Muolo, Emanuele Altomare – 1 May 1995 – The rate of mitochondrial oxidative phosphorylation and the cytosolic and mitochondrial total and oxidized glutathione concentrations were studied in regenerating rat livers after partial (70%) hepatectomy. The rate of mitochondrial oxidative phosphorylation progressively decreased during the early prereplicative phase of liver regeneration. This was accompanied by a progressive decrease in mitochondrial, but not cytosolic, glutathione concentration.

Bettina Grasl Kraupp, Branislav Ruttkay‐Nedecky, Helga Koudelka, Krystyna Bukowska, Wilfried Bursch, Rolf Schulte‐Hermann – 1 May 1995 – Detection of DNA fragments in situ using the terminal deoxyribonucleotidyl transferase (TDT)‐mediated dUTP‐digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate active cell death (apoptosis). We studied the specificity of the assay in well‐defined models of apoptosis and necrosis as well as in postmortem autolysis in rat liver.