Poa‐Chun Chang, Cheng‐Po Hu, Shu‐Hsia Chen, Sheng Wang‐Wuu, Chungming Chang – 1 June 1995 – We have previously reported the establishment of well‐differentiated BALB/c mouse liver (ML) cell lines. Transfection of these cell lines with hepatitis B virus (HBV) DNA led to the expression of HBV‐specific antigens and integration of HBV sequences in the cellular genome. Two cloned HBV‐transfected ML cell lines, ML‐2(HBV) and ML‐3(HBV), expressed viral antigens and were highly tumorigenic in nude mice. However, the tumorigenicity of the two cell lines was significantly reduced in BALB/c mice.

Chandan Guha, Motoki Osawa, Phillip A. Werner, Robert M. Galbraith, Gary V. Paddock – 1 June 1995 – Studies were performed in Hep3B hepatocytes to better elucidate the mechanisms regulating circulating levels of human group—specific component (Gc). We measured changes in Gc messenger RNA (mRNA) synthesis and levels of secreted protein resulting from treatment of hepatocytes with cytokines and hormones known to influence synthesis of other proteins of hepatic origin. We particularly focused on compounds known to be prototypic stimulants during the acute phase response.

Christine A. Wanke – 1 June 1995

Gloria Taliani, Franca Duca, Rosina Lecce, Donatella Livoli, Caterina Pasquazzi, Carlo De Bac, Andrea Fabbri, Giampaolo P. Bianchi, Guilio Marchesini – 1 June 1995

Shu Fukushima, Kazuo Honda, Masaaki Awane, Eiji Yamamoto, Ryouji Takeda, Ichiro Kaneko, Akira Tanaka, Taisuke Morimoto, Koichi Tanaka, Yoshio Yamaoka – 1 June 1995 – Using a polymerase chain reaction (PCR) method, we tested for the hepatic mitochondrial DNA (mtDNA) deletion in 40 hepatic tumors (28 hepatocellular carcinomas [HCCs], 9 other malignant tumors, and 3 benign tumors) and in the livers of 71 patients, including 16 pediatric patients with end‐stage liver disease who underwent living related donor liver transplantation and 16 liver donors.

Albrecht Guhlmann, Katja Krauss, Franz Oberdorfer, Thilo Siegel, Peter H. Scheuber, Juliane Müller, Brigitte Csuk‐Glänzer, Sibylle Ziegler, Hermann Ostertag, Dietrich Keppler – 1 June 1995 – N‐Acetyl‐leukotriene E4 has been identified as an endogenous, biologically less active cysteinyl leukotriene metabolite in rodents and humans. To evaluate the ratio of hepatobiliary to renal elimination of leukotrienes noninvasively by positron emission tomography (PET), we synthesized N‐[11C]acetyl‐leukotriene E4 by chemical N‐acetylation of leukotriene E4.

Sabine Angermüller, Marcus Schunk, Klaus Kusterer – 1 June 1995 – In the model of the perfused rat liver, we investigated the alterations of sinusoidal cells in the pathogenesis of liver injury caused by hypoxia and reperfusion. In sinusoidal endothelial cells, the activity of xanthine oxidase (XOX), a cytoplasmic marker enzyme, was located cytochemically and determined biochemically. Kupffer cells, identified by their endogenous peroxidase staining, were studied with regard to changes in their ultrastructure.

Maureen S. Thorniley, Sandra Simpkin, Barry Fuller, Mandana Z. Jenabzadeh, Colin J. Green – 1 June 1995 – Ischemia‐reperfusion injury is a major cause of transplant dysfunction. One feature of this damage is mitochondrial dysfunction.

Han Roelofsen, Conny T. M. Bakker, Berry Schoemaker, Marc Heijn, Peter L. M. Jansen, Ronald P. J. Oude Elferink – 1 June 1995 – The hepatocanalicular transport of a large number of organic anions, such as bilirubin glucuronides and glutathione conjugates in the rat, is mediated by an adenosine triphosphate (ATP)‐dependent transport system, which is termed canalicular multispecific organic anion transporter (cMOAT). This system is mainly defined by its deficiency in mutant TR− rats.

Dianzhong Luo, Karin Vanderkerken, Luc Bouwens, Peter J. K. Kuppen, Evelyne Crabbé, Eddie Wisse – 1 June 1995 – Pit cells are a unique population of cells in sinusoids and peripheral blood, which can be considered natural killer (NK) cells with large granular lymphocyte (LGL) morphology. The aim of this study was to investigate the use of the monoclonal antibody (MAb) 3.2.3 as a specific marker of rat pit cells to detect their number and distribution in the liver.