Naeem A. Essani, Michael A. Fisher, Anwar Farhood, Anthony M. Manning, C. Wayne Smith, Hartmut Jaeschke – 1 June 1995 – Neutrophil‐induced liver injury during endotoxemia is dependent on the adhesion molecule Mac‐1 (CD11b/CD18) on neutrophils. The potential involvement of its counterreceptor, intercellular adhesion molecule—1 (ICAM‐1), in the pathogenesis was investigated after administration of 100 μg/kg Salmonella abortus equi endotoxin (ET) in galactosamine‐sensitized mice (Gal).

Caryn M. Berkowitz, Cynthia S. Shen, Bahri M. Bilir, Edgardo Guibert, Jorge J. Gumucio – 1 June 1995 – Cholesterol 7α‐hydroxylase, the rate‐limiting enzyme in bile salt synthesis from cholesterol is a P450 enzyme (CYP7A). Its expression and activity are regulated by bile salts, cholesterol, hormones and a circadian modulator. Here we define the hepatocytes contributing to the expression of the rat CYP7A gene during its in vivo circadian variation.

Jorg Ziebell, Thomas A. Shaw‐Stiffel, Mitchell L. Shiffman – 1 June 1995

Poa‐Chun Chang, Cheng‐Po Hu, Shu‐Hsia Chen, Sheng Wang‐Wuu, Chungming Chang – 1 June 1995 – We have previously reported the establishment of well‐differentiated BALB/c mouse liver (ML) cell lines. Transfection of these cell lines with hepatitis B virus (HBV) DNA led to the expression of HBV‐specific antigens and integration of HBV sequences in the cellular genome. Two cloned HBV‐transfected ML cell lines, ML‐2(HBV) and ML‐3(HBV), expressed viral antigens and were highly tumorigenic in nude mice. However, the tumorigenicity of the two cell lines was significantly reduced in BALB/c mice.

Chandan Guha, Motoki Osawa, Phillip A. Werner, Robert M. Galbraith, Gary V. Paddock – 1 June 1995 – Studies were performed in Hep3B hepatocytes to better elucidate the mechanisms regulating circulating levels of human group—specific component (Gc). We measured changes in Gc messenger RNA (mRNA) synthesis and levels of secreted protein resulting from treatment of hepatocytes with cytokines and hormones known to influence synthesis of other proteins of hepatic origin. We particularly focused on compounds known to be prototypic stimulants during the acute phase response.

Christine A. Wanke – 1 June 1995

Gloria Taliani, Franca Duca, Rosina Lecce, Donatella Livoli, Caterina Pasquazzi, Carlo De Bac, Andrea Fabbri, Giampaolo P. Bianchi, Guilio Marchesini – 1 June 1995

Shu Fukushima, Kazuo Honda, Masaaki Awane, Eiji Yamamoto, Ryouji Takeda, Ichiro Kaneko, Akira Tanaka, Taisuke Morimoto, Koichi Tanaka, Yoshio Yamaoka – 1 June 1995 – Using a polymerase chain reaction (PCR) method, we tested for the hepatic mitochondrial DNA (mtDNA) deletion in 40 hepatic tumors (28 hepatocellular carcinomas [HCCs], 9 other malignant tumors, and 3 benign tumors) and in the livers of 71 patients, including 16 pediatric patients with end‐stage liver disease who underwent living related donor liver transplantation and 16 liver donors.

Albrecht Guhlmann, Katja Krauss, Franz Oberdorfer, Thilo Siegel, Peter H. Scheuber, Juliane Müller, Brigitte Csuk‐Glänzer, Sibylle Ziegler, Hermann Ostertag, Dietrich Keppler – 1 June 1995 – N‐Acetyl‐leukotriene E4 has been identified as an endogenous, biologically less active cysteinyl leukotriene metabolite in rodents and humans. To evaluate the ratio of hepatobiliary to renal elimination of leukotrienes noninvasively by positron emission tomography (PET), we synthesized N‐[11C]acetyl‐leukotriene E4 by chemical N‐acetylation of leukotriene E4.

Sabine Angermüller, Marcus Schunk, Klaus Kusterer – 1 June 1995 – In the model of the perfused rat liver, we investigated the alterations of sinusoidal cells in the pathogenesis of liver injury caused by hypoxia and reperfusion. In sinusoidal endothelial cells, the activity of xanthine oxidase (XOX), a cytoplasmic marker enzyme, was located cytochemically and determined biochemically. Kupffer cells, identified by their endogenous peroxidase staining, were studied with regard to changes in their ultrastructure.