In situ detection of fragmented dna (tunel assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: A cautionary note

Bettina Grasl Kraupp, Branislav Ruttkay‐Nedecky, Helga Koudelka, Krystyna Bukowska, Wilfried Bursch, Rolf Schulte‐Hermann – 1 May 1995 – Detection of DNA fragments in situ using the terminal deoxyribonucleotidyl transferase (TDT)‐mediated dUTP‐digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate active cell death (apoptosis). We studied the specificity of the assay in well‐defined models of apoptosis and necrosis as well as in postmortem autolysis in rat liver.

The enigma of anti‐neutrophil antibodies in ulcerative colitis primary sclerosing cholangitis: Important genetic marker or epiphenomenon?

Roger W. Chapman – 1 May 1995 – Background/Aims: Perinuclear antineutrophil cytoplasmic antibodies (pANCA) were found at high frequency in patients with primary sclerosing cholangitis and ulcerative colitis. In this study, to accumulate further evidence for the importance of genetic factors in pathogenesis of inflammatory bowel disease, sera of patients with inflammatory bowel disease and primary sclerosing cholangitis and their unaffected family members were tested for pANCA.

Alterations in bile ducts and peribiliary microcirculation in rats after common bile duct ligation

Shu Nakano, Joji Haratake, Hiroshi Hashimoto – 1 May 1995 – The chronological changes in ductular epithelium and peribiliary capillary plexuses (PBPs) after bile duct ligation are not well understood. Therefore, we examined alterations in both intrahepatic bile ductules and peribiliary microcirculation in rats after ligation of the common bile duct using immunohistochemistry, transmission electron microscopes (TEM), and scanning electron microscopes (SEM).

Role of cytoskeleton and acidification of endocytic compartment in asialoglycoprotein metabolism in isolated rat hepatocyte couplets

Masaru Harada, Shotaro Sakisaka, Masao Yoshitake, Masahito Ohishi, Satoshi Itano, Satoshi Shakado, Yoshihiro Mimura, Kazunori Noguchi, Michio Sata, Hiroshi Yoshida, Kyuichi Tanikawa – 1 May 1995 – The process of receptor‐mediated endocytosis is common to a variety of species and cell types. One of the best characterized receptor‐ligand systems is the hepatocyte receptor for asialoglycoproteins. We investigated the morphological features of the uptake and intracellular transport of gold‐conjugated asialofetuin in isolated rat hepatocyte couplets.

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