Transferrin receptors and the uptake and release of iron by isolated hepatocytes

Stephen P. Young, Philip Aisen – 1 March 1981 – Isolated rat hepatocytes accumulate iron from iron‐transferrin by a process which is dependent on the temperature and on the transferrin concentration, and which is diminished by treatment of the cells with a proteolytic enzyme. These observations are consistent with a mechanism for iron uptake into hepatocytes involving the binding of iron‐transferrin to a specific cell‐surface receptor.

In vitro effect of bile salts on rat liver plasma membrane, lipid fluidity, and ATPase activity

Bruce F. Scharschmidt, Emmet B. Keeffe, Donald A. Vessey, Nancy M. Blankenship, Robert K. Ockner – 1 March 1981 – Considerable evidence suggests that liver plasma membrane (LPM) NaK‐ATPase [(Na+ + K+)‐dependent adenosinetriphosphatase] and Mg‐ATPase (Mg2+‐dependent adenosinetriphosphatase) activity and lipid fluidity are important in liver cell functions such as bile formation. However, little is known regarding factors which might alter these membrane properties in vivo.

Urinary Coproporphyrin isomers in Rotor's syndrome: A study in eight families

Yoshitaka Shimizu, Hiroshi Naruto, Setsuko Ida, Masateru Kohakura – 1 March 1981 – Urinary coproporphyrin isomers were measured in 17 patients with Rotor's syndrome, 65 phenotypically normal relatives of 14 patients from eight families, and 21 normal subjects. Coproporphyrin I was elevated in Rotor's syndrome (p < 0.001) and, to a lesser degree, in phenotypically normal parents (p < 0.005), children, and siblings (p < 0.025) as compared to normal controls. Coproporphyrin III in patients, parents, and children did not differ from that in normal controls.

Effects of corticosteroids on bilirubin metabolism in patients with Gilbert's syndrome

Hideki Ohkubo, Kunio Okuda, Shinji Iida – 1 March 1981 – To elucidate the mechanism whereby corticosteroids decrease the serum bilirubin concentration, changes in bilirubin metabolism were studied in patients with Gilbert's syndrome using a bilirubin load test and/or nicotinic acid test before and after corticosteroid treatment. Steroid administration increased hepatic clearance and uptake of bilirubin; the transfer rate for biliary excretion was unaffected.

Modulation of the transport of bilirubin and asialoorosomucoid during liver regeneration

Ulrich Gärtner, Richard J. Stockert, Anatol G. Morell, Allan W. Wolkoff – 1 March 1981 – The normal rat hepatocyte divides approximately once per year but, following two thirds hepatectomy, rapid cellular replication occurs throughout the remaining liver remnant. Using a multiple indicator dilution technique, single‐pass transport of 3H‐bilirubin and 125I‐asialoorosomucoid was studied in isolated perfused liver from 6hr to 6d after two thirds hepatectomy or sham surgery. Influx (k1), efflux (k2), and sequestration (k3) rates were quantitated by computer analysis.

HLA typing in idiopathic hemochromatosis: Distinction between homozygotes and heterozygotes with biochemical expression

Mark L. Bassett, June W. Halliday, Lawrie W. Powell – 1 March 1981 – In a study of 20 families with idiopathic hemochromatosis, relatives of probands were classified as either homozygous, heterozygous, or normal according to their HLA phenotype. An abnormality in the serum iron concentration, total iron‐binding capacity, or serum ferritin concentration was present in all homozygotes and in 25% of heterozygotes. In heterozygotes, the mean total iron‐binding capacity was significantly decreased, and the mean hepatic iron concentration was significantly increased compared to normals.

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