A low molecular weight binding protein for organic anions (Z protein) from human hepatic cytosol: Purification and quantitation

Kazuaki Kamisaka, Hidenori Maezawa, Tooru Inagaki, Kenichi Okano – 1 May 1981 – Human Z protein from liver was purified to homogeneity. The protein has a molecular weight of 11,000 and an isoelectric point of pH 5.8. Circular dichroism spectra of Z protein‐bilirubin (uncon‐jugated and diglucuronide) complexes revealed two ellipticity extrema, a negative peak at 460 nm, and a positive peak at 410 nm. Human serum albumin had a higher affinity for bilirubin than did Z protein.

Hepatic lysosomal copper protein in dogs with an inherited copper toxicosis

Gerald F. Johnson, Anatol G. Morell, Richard J. Stockert, Irmin Sternlieb – 1 May 1981 – Hepatic copper overload inherited as an autosomal recessive trait in Bedlington Terriers is characterized by the presence of hepatocellular lysosomal granules of unusually high specific gravity and electron density which contain at least two thirds of the total hepatic copper.

Structural heterogeneity of hepatocyte “tight” junctions: A quantitative analysis

Suzanne Lagarde, Elwyn Elias, James B. Wade, James L. Boyer – 1 May 1981 – Freeze fracture analyses of hepatocyte tight junctions reveal interconnecting strands which represent a semipermeable blood‐bile barrier. In epithelia, strand number can be correlated with transepithelial resistance and junctional permeability, but a functional relationship is not always seen. We have devised an analytical method to measure accurately strand number as well as junction depth over a large area of junction.

A noncollagenous matrix for attachment of rat hepatocytes in culture

Patricia Ponce, Javier Cordero, Marcos Rojkind – 1 May 1981 – Adult rat hepatocytes efficiently attach to intact connective tissue fibers prepared from normal rat liver; this material has been given the name of “biomatrix”. The cells remain alive and differentiated for at least 4 months in culture. The liver biomatrix contains both collagen and noncollagenous glycoproteins. Heretofore, the functions of the different components of the biomatrix in facilitating cell adhesion and promoting survival and differentiation of rat hepatocytes have not been investigated.

Antiviral treatment of chronic hepatitis B virus infection: Improvement in liver disease with interferon and adenine arabinoside

George H. Scullard, Ljudevit L. Andres, Harry B. Greenberg, Joseph L. Smith, Vinod K. Sawhney, E. Andrew Neal, Anmol S. Mahal, Hans Popper, Thomas C. Merigan, William S. Robinson, Peter B. Gregory – 1 May 1981 – Twelve of 32 patients lost HBeAg and DNA polymerase from their serum after completing antiviral therapy with interferon and/or adenine arabinoside. Three lost serum HBsAg as well. Loss of corresponding viral markers from hepatic tissue was also seen.

Presence and meaning of anti‐HBc IgM as determined by ELISA in patients with acute type B hepatitis and healthy HBsAg carriers

Peter Kryger, Lars R. Mathiesen, Jan Aldershvile, Jens O. Nielsen, The Copenhagen Hepatitis Acuta Programme – 1 May 1981 – To evaluate the clinical implication of IgM antibody against hepatitis B core antigen (anti‐HBc IgM), a consecutive series of 87 patients with acute type B hepatitis and 16 healthy carriers of hepatitis B surface antigen (HBsAg) were studied by a newly developed reverse ELISA technique.

Subscribe to