Kim A. Sathre, Monica L.‐S. Tsang, James A. Weatherbee, Clifford J. Steer – 1 August 1991 – Cellular processing of 125I‐labeled transforming growth factor‐β1 was investigated in the human hepatoma cell lines Hep G2 and Hep 3B. Binding of 125I‐transforming growth factor‐β1 to cell surface receptors was specific, saturable and calciumindependent. Both cell lines exhibited a single class of high‐affinity (Kd = 2.2 × 10−10 mol/L) binding sites (4.5 × 103 for the Hep G2 cell; 1.5 × 103 for the Hep 3B cell) for both human and porcine transforming growth factor‐β1.

Miriam J. Alter – 1 August 1991

Daniel W. Nebert – 1 August 1991 – These two reports describe recombinant DNA tests that can identify individuals having a defect in the cytochrome P450IID6 (CYP2D6)‐mediated oxidative metabolism of debrisoquine and more than two dozen other drugs that are commonly prescribed. The poor metabolizer (PM), representing 5% to 10% of the northern European white population is homozygous for an autosomal recessive trait. Compared with the extensive metabolizer (EM) phenotype, the PM individual is more prone to toxicity caused by some of these drugs.

John A. Hermos – 1 August 1991

Raymond S. Koff – 1 August 1991

Jean‐François Bretagne, Jean‐Luc Raoul, Michel Gosselin, Kunio Okuda, Kunio Kimura – 1 August 1991

Bertram I. Cohen, Nariman Ayyad, Erwin H. Mosbach, Charles K. McSherry, Naoyuki Matoba, Alan F. Hofmann, Huong‐Thu Ton‐Nu, Ying Peng, Claudio D. Schteingart, Richard J. Stenger – 1 July 1991 – The effect of two hydrophilic bile acids, murideoxycholic acid (3α,6β‐dihydroxy‐5β‐cholanoic acid) and ursodeoxycholic acid, on cholesterol and bile acid metabolism and hepatic pathology and gallstone composition was studied in the prairie dog. Cholesterol gallstones were induced by feeding a diet containing 1.2% cholesterol for 75 days.

Emilio Barberá‐Guillem, Marian Rocha, Antonia Alvarez, Fernando Vidal‐Vanaclocha – 1 July 1991 – We have studied the distribution patterns of carbohydrate terminals on the endothelial surface of the mouse liver microvasculature. For this purpose, a wide battery of FITC lectins specific to glucose, mannose, galactose, fucose, N‐acetyl‐neuraminic acid, N‐acetylgalactosamine and N‐acetyl‐glucosamine residues were incubated on liver cryostat sections or intraportally perfused under physiological conditions.

Rebecca W. van Dyke – 1 July 1991 – We have used retrovirus‐mediated gene transfer to demonstrate complementation of the cystic fibrosis (CF) defect in vitro. Amphotropic retroviruses were used to transduce a functional cystic fibrosis transmembrane conductance regulator (CFTR) cDNA into CFPAC‐1, a pancreatic adenocarcinoma cell line derived from a patient with CF that stably expresses the chloride transport abnormalities characteristic of CF.

Harold O. Conn – 1 July 1991 – Nine thousand two hundred twelve liver biopsies were performed according to a defined protocol, and data were prospectively recorded to identify risk factors for major bleeding. There were 10 fatal and 22 nonfatal hemorrhages (0.11% and 0.24%, respectively). By comparison with a control group that did not hemorrhage, malignancy, age, sex, and the number of passes were the only predictable risk factors. The risk of fatal hemorrhage in patients with malignancy is estimated to be 0.4%; for nonfatal hemorrhage, 0.57%.