Efficient silencing of hepatitis B virus S gene through CRISPR‐mediated base editing

Hao Zhou, Xiaomei Wang, Clifford J. Steer, Guisheng Song, Junqi Niu – 25 March 2022 – Hepatitis B virus (HBV) infection is a major risk factor of liver cirrhosis and hepatocellular carcinoma. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) has been used to precisely edit the HBV genome and eliminate HBV through non‐homologous end‐joining repair of double‐stranded break (DSB). However, the CRISPR/Cas9‐mediated DSB triggers instability of host genome and exhibits low efficiency to edit genome, limiting its application.

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