Alteration in vascular reactivity in isolated aortic rings from portal vein—constricted rats

Stelios Karatapanis, P. Aiden McCormickM.D.MRCP, Shilpa Kakad, Jason K. T. Chin, Mohammed Islam, Jamie Jeremy, David Harry, Neil McIntyre, Andrew K. Burroughs, Michael Jacobs – 1 December 1994 – It has been suggested that increased production of nitric oxide by an inducible nitric oxide synthase isoenzyme is important in the pathogenesis of the vascular abnormalities seen in human beings and animals with portal hypertension. We investigated this hypothesis by studying the in vitro vascular reactivity of isolated aortic rings from portal vein—constricted and sham‐operated rats.

Identifying the hepatic organic anion transporter: One of many?

Steven A. Weinman – 1 December 1994 – Using expression cloning in Xenopus laevis oocytes, we have isolated a cDNA encoding a rat liver organic anion‐transporting polypeptide (oatp). The cloned oatp mediated Na+‐independent uptake of sulfobromophthalein (BSP) which was Cl−‐dependent in the presence of bovine serum albumin (BSA) at low BSP concentrations (e.g., 2 μM). Addition of increasing amounts of BSA had no effects on the maximal velocity of initial BSP uptake, but it increased the Km value from 1.5 μM (no BSA) to 24 μM (BSA/BSP molar ratio, 3.7) and 35 μM (BSA/BSP ratio, 18.4).

FK 506 renal toxicity and lack of detectable cytochrome P‐450 3A in the liver graft of a patient undergoing liver transplantation

Antoinette Lemoine, Daniel Azoulay, Ashley Dennison, Liliane Kiffel, Lydiane Pichard, Valérie Furlan, Thierry Bienvenu, Gilles Fredj, Brigitte Debuire, Patrick Maurel, Henri Bismuth, Philippe Beaune – 1 December 1994 – Many commonly used drugs are substrates for hepatic cytochrome P‐450 3A in human beings, and its role in the metabolism of potentially toxic immunosuppressants has been highlighted (cyclosporine, FK 506).

Effects of obesity on bile formation and biliary lipid secretion in the genetically obese JCR:LA‐corpulent rat

Catherine M. St. George, James C. Russell, Eldon A. Shaffer – 1 December 1994 – Obesity, a known risk factor in cholesterol gallstone disease, is a favorable factor in the formation of bile supersaturated with cholesterol. Previous studies have been indirect and limited to human beings. To better define the hepatic secretory defect, we directly measured bile secretion and bile salt kinetics in a genetically obese rat.

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