Uw‐preservation of cultured human gallbladder epithelial cells: Phenotypic alterations and differential mucin gene expression in the presence of bile
Jean‐Pierre Campion, Nicole Porchet, Jéan‐Pierre Aubert, Annie L'Helgoualch, Bruno Clément – 1 January 1995 – In orthotopic liver transplantation, extended cold ischemia of the graft may induce cell damage, particularly in biliary epithelium. We have investigated the effects of a cold University of Wisconsin (UW) solution on cultured human gallbladder biliary epithelial cells (GBEC) exposed or not exposed to stagnant bile. In UW solution, morphological alterations of cultured GBEC were not prominent under light microscopy after 16 hours at 4°C, being more striking after 24 to 48 hours.